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qiagen質(zhì)粒大量提取試劑盒12162 12163

QIAGEN Plasmid Kit procedures.

Neutralized bacterial lysates are cleared by centrifugation. The cleared lysate is then loaded onto the anion-exchange tip where plasmid DNA selectively binds under appropriate low-salt and pH conditions. RNA, proteins, metabolites, and other low-molecular-weight impurities are removed by a medium-salt wash, and ultrapure plasmid DNA is eluted in high-salt buffer. The DNA is concentrated and desalted by isopropanol precipitation and collected by centrifugation.

qiagen質(zhì)粒大量提取試劑盒12162 12163

Performance

QIAGEN Plasmid Kit利用重力流QIAGEN陰離子交換柱技術(shù)高效純化質(zhì)粒DNA。產(chǎn)量可達(dá)10 mg（Giga）、2.5 mg（Mega）、500 µg（Maxi）、或100 µg（Midi）高拷貝質(zhì)粒DNA（培養(yǎng)體積取決于質(zhì)粒拷貝數(shù)、插入片段大小、宿主菌株和培養(yǎng)基。）QIAGEN Plasmid Kits純化的質(zhì)粒DNA適合多種應(yīng)用

訂購信息：

12162質(zhì)粒大量提取試劑盒QIAGEN Plasmid Maxi Kit (10) 規(guī)格：10次

12163質(zhì)粒大量提取試劑盒QIAGEN Plasmid Maxi Kit (25) 規(guī)格：25次