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ERK 2 （C-14） 抗體

ERK 2 Background Information
Mitogen-activated protein kinase （MAPK） signaling pathways involve two closely related MAP kinases, known as extracellular-signal-related kinase 1 （ERK 1, p44） and 2 （ERK 2, p42）. Growth factors, steroid hormones, G protein-coupled receptor ligands, and neurotransmitters can initiate MAPK signaling pathways. Activation of ERK1 and ERK2 requires phosphorylation by upstream kinases such as MAP kinase kinase （MEK）, MEK kinase and Raf-1. ERK1 and ERK2 phosphorylation can occur at specific tyrosine and threonine sites mapping within consensus motifs that include the Threonine-Glutamate-Tyrosine motif. ERK activation leads to dimerization with other ERKs and subsequent localization to the nucleus. Active ERK dimers phosphorylate serine and threonine residues on nuclear proteins and influence a host of responses that include proliferation, differentiation, transcription regulation and development. The human ERK2 gene maps to chromosome 22q11.21 and encodes a 360-amino acid protein.

• rabbit polyclonal IgG, 200 µg/ml; also available as goat IgG, 200 µg/ml, sc-154-G
• epitope mapping at the C-terminus of ERK 2 of rat origin
• recommended for detection of ERK 2 p42 and, to a lesser extent, ERK 1 p44 of mouse, rat, human, avian, frog, zebrafish and Xenopus origin by WB, IP, IF, IHC（P）, FCM and ELISA; also reactive with additional species, including equine, canine, bovine, porcine and avian
• blocking peptide, sc-154 P
• agarose conjugate for IP studies, sc-154 AC, 500 µg/0.25 ml agarose
• fluorescein （sc-154 FITC） and rhodamine （sc-154 TRITC） conjugates for immunofluorescence, 200 µg/ml
• phycoerythrin （sc-154 PE） conjugate for intracellular FCM, 100 tests
• Alexa Fluor® 405 （sc-154 AF405）, Alexa Fluor® 488 （sc-154 AF488） and Alexa Fluor® 647 （sc-154 AF647） conjugates for FCM or immunofluorescence; 100 µg/2 ml

ERK 2 （C-14）: sc-154. Western blot analysis of ERK 2 expression in A-431 （A）, HeLa （B）, KNRK （C）, NIH/3T3 （D）, NRK （E） and DU 145 （F） whole cell lysates.

ERK 2 （C-14）-G: sc-154-G. Western blot analysis of ERK 1 and ERK 2 expression in A-431 （A）, HeLa （B）, KNRK （C） and NIH/3T3 （D） whole cell lysates.

ERK 2 （C-14）: sc-154. Immunofluorescence staining of methanol-fixed HeLa cells （A） and immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue （B） showing cytoplasmic localization.

Western blot analysis of ERK in 3611-RF （A,D）, NIH/3T3 （B,E） and KNRK （C,F） whole cell lysates. Antibodies tested include ERK 1 （K-23）-G: sc-94-G （A-C） and ERK 2 （C-14）-G: sc-154-G （D-F）.

ERK 2 （C-14） Alexa Fluor 488: sc-154 AF488. Intracellular FCM analysis of fixed and permeabilized HeLa cells. Black line histogram represents the isotype control, normal rabbit IgG: sc-45068.

ERK 2 （C-14） Alexa Fluor 647: sc-154 AF647. Intracellular FCM analysis of fixed and permeabilized HeLa cells. Black line histogram represents the isotype control, normal rabbit IgG: sc-24647.

ERK 2 （C-14）: sc-154. Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization using indirect FITC （A） staining and direct Alexa Fluor 488 （B） staining.

ERK 2 （C-14）: sc-154. Western blot analysis of ERK 1 expression in non-transfected 293T: sc-117752 （A）, mouse ERK 1 transfected 293T: sc-126806 （B） and A-431 （C） whole cell lysates.

ERK 2 （C-14）: sc-154. Western blot analysis of ERK 2 expression in rat hippocampus tissue extract.

ERK 2 （C-14）-G: sc-154-G. Western blot analysis of ERK 2 expression in rat hippocampus tissue extract.

ERK 2 （C-14） PE: sc-154 PE. Intracellular FCM analysis of fixed and permeabilized NIH/3T3 cells. Black line histogram represents the isotype control, normal rabbit IgG: sc-3871.

ERK 2 （C-14）-G: sc-154-G. Western blot analysis of ERK 2 expression in non-transfected 293T: sc-117752 （A）, human ERK 2 transfected 293T: sc-177196 （B）, A-431 （C） and HeLa （D） whole cell lysates.

ERK 1 （C-14）: sc-154. Western blot analysis of ERK 1 expression in non-transfected: sc-117752 （A） and human ERK 1 transfected: sc-171765 （B） 293T whole cell lysates.

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